what do you make of:
https://pubmed.ncbi.nlm.nih.gov/30226803/#:~:text=Rabies virus is shed in,in the US each year.
Isolation of Rabies Virus from the Salivary Glands of Wild and Domestic Carnivores during a Skunk Rabies Epizootic
Isabel Jimenez 1, Terry Spraker 2, Jessica Anderson 3, Richard Bowen 3, Amy Gilbert 4
- PMID: 30226803
- DOI: 10.7589/2018-05-127
Rabies is a fatal zoonotic disease of global importance. Rabies virus is shed in the saliva of infected hosts and is primarily transmitted through bite contact. Canine rabies has been eliminated from the US, but wildlife constitutes more than 90% of the reported cases of animal rabies in the US each year.
Unfortunately, we don't have the full paper, but only the abstract. So we don't know exactly what methodology was employed. Hence nothing can be said about whether or not the rabies virus was isolated in the true sense of the word.
To illustrate, here is another (this time full) paper:
The title of this latter paper ("ISOLATION OF RABIES VIRUS FROM THE PAROTID SALIVARY GLANDS OF FOXES") also suggests that viruses were isolated: but they weren't. The macerated and diluted salivary gland tissues of sick animals, along with antibiotics and rabbit serum, were injected into healthy mice. Then those mice that died were tested using FAT (Fluorescent Antigen Test) and something called MIT -- which acronym I can't find expanded. Since this was positive in some of the mice, they took it that the virus was present.
They didn't first isolate the virus in the sense of actually separating it from saliva and confirming that with electronmicroscopy. They didn't inject such a true isolate into the brain of healthy mice, but rather macerated/diluted salivary gland material along with antibiotics and rabbit serum. As far as I can see, they didn't perform a control experiment where pure distilled water plus antibiotics/rabbit serum MINUS the salivary gland extract was injected to see whether that too might cause the death of mice.
In this paper, just using the word "isolation" doesn't mean actual isolation occurred. Since I can't see what was done in the paper you posted the abstract of, I can't make a specific judgement of what they did. But if it was something similar, then my comments would be the same.
Have you by any chance read the full paper? If so, could you kindly let us know what experimental methodology was applied? Unless there was true isolation, and the absence of antibiotics/animal serum, along with electronmicroscopy of a portion of the same isolate, one can't say that the virus had been isolated. And, of course, controls would be needed to rule out the possibility that injecting distilled water, antibiotics and animal serum alone caused the same effect as with the parotid gland extract.
The antigens tested for in the example I've provided presumably came from some earlier experiment. One would have to find an example of that and determine whether true isolation occurred in that instance. That's key, and if one can find it, then the case is much stronger. So far, I haven't been able to track such a paper down. I'm not alone -- others have tried and not been able to find a single case where true isolation was performed for any virus.
Incidentally, FOIAs from many medical authorities have confirmed in writing that no true isolation of any virus has been performed.